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KMID : 0829220100340060331
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Korean Journal of Oral and Maxillofacial Pathology
2010 Volume.34 No. 6 p.331 ~ p.337
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Urokinase Type Plasminogen Activator and Plasminogen Aativator Inhibitor-1 Expression In Oral Squamous Cell Carcinoma Cell Line
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Yu Yeon-Sang
Chun Yoon-Kwon
Park Gyeong-Ju
Lee Chong-Heon
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Abstract
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Tumor cell biological factors, such as urokinase plasminogen activator(uPA) and its inhibitor plasminogen activator inhibitor-1(PAI-1) play a role in tumor invasion, metastasis, and proliferation. These factors in patients with primary oral squamous cell carcinoma(Oral SCC) will be evaluated and correlated with clinicopathologic variables. However, relatively rarely has been known in oral squamous cell carcinoma in vivo and in vitro study. The purpose of this study were to investigate the protein expression of uPA and PAI-1 in oral SCC cell lines cell line compared to NHOK and to study migration and adhesion assay. All the cell lines were cultured under KBM bullet kit at 37℃ in a 5% CO2 incubator. We studied a possible association between cytosolic uPA and PA-1 concentrations in oral SCC cell line compared to NHOK using an enzyme-linked immunoassay(ELISA). Cell adhesion and migration assay were done in all the cell lines. In migration assay oral SCC cell lines were about 70 folds higher than NHOK. In adhesion assay oral SCC cell line were about 7-12 folds higher than NHOK. uPA cytosolic concentrations was about 15-19 folds and PAI-1 was 3 to 4.5 folds than that of NHOK. Both uPA and PAI-1 concentrations were correlated with migration and adhesion assay. High cytosolic concentrations of uPA and PAI-1 were correlated with migration and adhesion assay. It suggested that these markers might be specific for oral SCC cell line and these results would be contributed to treatment and prognosis of human oral squamous cell carcinoma.
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KEYWORD
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UPA, PAI-1 Expression, Oral SCC Cell Line
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